![]() ![]() ![]() For U-47700 (74.5–547 ng/mL) and furanyl fentanyl ( CONCLUSIONS: The MOR reporter assay allows rapid identification of opioid activity in blood. Hence, further evaluation did not include these samples. RESULTS: In 8 samples containing synthetic opioids, in which no positive signal was obtained in the bioassay, quadrupole time-of-flight mass spectrometry revealed the MOR antagonist naloxone, which can prevent receptor activation. Following evaporation and reconstitution in 100 μL of Opti-MEM ® I, 20 μL was analyzed in the bioassay. Blood (500 μL) was extracted via solid-phase extraction. Assay performance was evaluated on 107 postmortem blood samples. ![]() METHODS: A cell-based system was set up in which activation of the μ-opioid receptor (MOR) led to recruitment of β-arrestin 2, resulting in functional complementation of a split NanoLuc luciferase and allowing readout via bioluminescence. Here we present a novel screening method for the detection of opiates and (synthetic) opioids based on their activity. Abstract: BACKGROUND: Detection of new highly potent synthetic opioids is challenging as new compounds enter the market.
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